ReportBio RA1002 Anti-Beta Actin Mouse Monoclonal Antibody

Beta-actin is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. Beta-actin has been shown to interact with SPTBN2. In addition, RNA-binding protein Sam68 was found to interact with the mRNA encoding β-actin, which regulates the synaptic formation of the dendritic spines with its cytoskeletal components. Beta-actin has been shown to activate eNOS, thereby increasing NO production. An eight-amino acid residue (326-333) in actin has been shown to mediate the interaction between actin and eNOS. Recurrent mutations in this gene have been associated to cases of diffuse large B-cell lymphoma. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.

产品名称     Anti-Beta Actin Mouse Monoclonal Loading control Antibody [A2-F6]

分子量        42 kDa

种属反应性 Human,Mouse,Rat

验证应用    WB,ICC,IHC-P,FC

抗体类型    小鼠单抗

免疫原       Synthetic peptide (KLH-coupled) within human Beta-actin N terminal.

偶联         Non-conjugated

形态         Liquid

存放说明   Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型            IgG1

纯化方式     Protein A affinity purified.

亚细胞定位 Cytoplasm.